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EPICENTRE's revolutionary Nextera™ technology* uses in vitro transposition to prepare sequencer-ready libraries from genomic DNA for multiple sequencing platforms. The technology simultaneously fragments and tags DNA, in a single-tube reaction.
- Use nanogram amounts of starting DNA.
- Prepare sequencer-ready libraries in less than 2 hours.
- Incorporate platform-specific tags and optional barcodes.
- Validated on Roche 454™ GS FLX and GS FLX Titanium™, and Illumina Solexa® GAI, GAII.
| Fragmentation |
(15-30 min) |
Add Nextera™ Enzyme Mix
(5 min)
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| Collection |
(15 min) |
| Concentration |
(15 min) |
| Size Selection |
(60 min) |
| End-Repair |
(60 min) |
| Clean-Up |
(15 min) |
| A-Tailing |
+/- (30 min) |
| Adaptor Ligation |
(60 min) |
| Clean-Up |
(15 min) |
(15 min) |
| Library Enrichment |
Variable (~60 min) |
PCR (~60 min) |
For more information:
Product Information and ordering:
DNA Sequencing |
